Product ID:SAFRANIN COUNTER STAIN FOR GRAM METHOD, 15-4724A
MSDS Date:01/02/1998
FSC:6810
NIIN:00000000000
Status Code:A
MSDS Number: CKBSW

CAROLINA BIOLOGICAL SUPPLY CO

2700 YORK RD
BURLINGTON, NC 27215-3387
US

Emergency Phone: 800-227-1150

Info Phone: 800-227-1150

Cage: 59896

CAROLINA BIOLOGICAL SUPPLY CO

BURLINGTON, NC 27215-3387 US

Telephone: 800-227-1150/910-584-0381

Cage: 59896

SAFRANIN

CAS: 477-73-6

RTECS: SG1623000

ETHYL ALCOHOL

CAS: 64-17-5

RTECS: KQ6300000

OSHA PEL 1000 PPM

ACGIH TLV: 50 PPM

METHYL ISOBUTYL KETONE (DENATURANT)

CAS: 108-10-1

RTECS: SA9275000

OSHA PEL 50 PPM

ACGIH TLV: 50 PPM

EPA Report Quantity: 5000 LBS

DOT Report Quantity: 5000 LBS

HEXANE (DENATURANT)

CAS: 110-54-3

RTECS: MN9275000

OSHA PEL 500 PPM

ACGIH TLV: 50 PPM

EPA Report Quantity: 1 LB

DOT Report Quantity: 1 LB

LD50 LC50 Mixture:LD50 (ORAL, RAT): 7,060 MG/KG (ETHANOL)
Routes of Entry: Inhalation:YES Skin:NO Ingestion:NO
Reports of Carcinogenicity:NTP:NO IARC:NO OSHA:NO
Health Hazards Acute and Chronic:TARGET ORGANS: EYES, SKIN, NOSE.
ACUTE: EYES: MAY CAUSE IRRITATION. SKIN: MAY CAUSE IRRITATION.
INHALATION: MAY CAUSE IRRITATION TO RESPIRATORY TRACT. INGESTION:
MAY CAUSE GASTROINTESTINAL DISCOMFORT.
Explanation of Carcinogenicity:EACH OF THE FOUR INGREDIENTS ARE NOT
LISTED AS CAUSING CANCER BY IARC, NTP, OSHA.
Effects of Overexposure:MAY CAUSE IRRITATION TO EYES, SKIN, AND MUCOUS
MEMBRANES UNDER PROLONGED EXPOSURE. IF SWALLOWED, MAY CAUSE
GASTROINTESTINAL IRRITATION, NAUSEA, VOMITING, DIARRHEA.
Medical Cond Aggravated by Exposure:PERSONS WITH PRE-EXISTING
CONDITIONS OF THE EYES, SKIN, AND NOSE MAY BE MORE SUSCEPTIBLE TO
THE EFFECTS OF THIS PRODUCT. TARGET ORGANS: EYES, SKIN, NOSE.

First Aid:EYES: FLUSH WITH WATER FOR AT LEAST 15 MINUTES, RAISING AND
LOWERING EYELIDS. GET MEDICAL ATTENTION IF IRRITATION PERSISTS.
SKIN: WASH EXPOSED AREA FOR AT LEAST 15 MINUTES. REMOVE
CONTAMINATED CLOTHIN G AND LAUNDER BEFORE REUSE. GET MEDICAL
ATTENTION IF IRRITATION PERSISTS. INHALATION: MOVE TO FRESH AIR.
GIVE OXYGEN IF BREATHING IS DIFFICULT. GIVE ARTIFICIAL RESPIRATION
IF BREATHING HAS STOPPED. GE T MEDICAL ATTENTION. INGESTION: DO NOT
INDUCE VOMITING. IF SWALLOWED, IF CONSCIOUS, GIVE PLENTY OF WATER
IMMEDIATELY AND CALL A PHYSICIAN OR POISON CONTROL CENTER. NEVER
GIVE ANYTHING BY MOUTH TO AN U NCONSCIOUS PERSON.

Flash Point Method:CC
Flash Point:=13.1C, 55.6F
ETHYL ALCOHOL
Autoignition Temp:=422.8C, 793.F
Autoignition Temp Text:E. ALC
Lower Limits:3.3 ETHANOL
Upper Limits:19 ETHANOL
Extinguishing Media:USE MEDIA SUITABLE TO EXTINGUISH SURROUNDING FIRE.
Fire Fighting Procedures:FIREFIGHTERS SHOULD WEAR FULL PROTECTIVE
EQUIPMENT AND NIOSH APPROVED SELF-CONTAINED BREATHING APPARATUS.
Unusual Fire/Explosion Hazard:FLAMMABLE VAPORS MAY BE PRESENT, IF
PRODUCT IS HEATED, WHICH MAY IGNITE IF EXPOSED TO HEAT, SPARKS, AND
OPEN FLAME. MFRS MSDS SHOWS NFPA RATING FOR PRODUCT AS "FIRE 2".
RATING IS FOR LIQUID MATERIAL WITH A FLASH POINT ABOVE 100 F, BUT
NOT EXCEEDING 200F. HCC FOR 100F F. P. TO 141F IS F4; FROM 141F TO
200F, HCC IS V4.

Spill Release Procedures:VENTILATE AREA OF SPILL. ELIMINATE ALL SOURCES
OF IGNITION. REMOVE ALL NON-ESSENTIAL PERSONNEL FROM AREA. CLEAN-UP
PERSONNEL SHOULD WEAR PROPER PROTECTIVE EQUIPMENT AND CLOTHING.
ABSORB MATERIAL WITH SUITABLE ABSORBANT AND CONTAINERIZE FOR
DISPOSAL.

Handling and Storage Precautions:THIS MATERIAL SHOULD BE KEPT IN AN
AREA SUITABLE FOR THE STORAGE OF FLAMMABLE LIQUIDS. BOND AND GROUND
CONTAINERS WHEN TRANSFERRING LIQUID.
Other Precautions:AVOID BREATHING VAPORS. AVOID EYE OR SKIN CONTACT.
WASH THOROUGHLY AFTER HANDLING AND BEFORE EATING OR DRINKING. KEEP
OUT OF REACH OF CHILDREN.

Respiratory Protection:A NIOSH/MSHA CHEMICAL CARTRIDGE RESPIRATOR
SHOULD BE WORN IF PEL OR TLV IS EXCEEDED.
Ventilation:MECHANICAL (GENERAL) AND/OR LOCAL EXHAUST.
Protective Gloves:RUBBER, NEOPRENE, PVC, OR EQUIVALENT.
Eye Protection:SPLASH PROOF CHEMICAL SAFETY GOGGLES SHOULD BE WORN AT
ALL TIMES.
Other Protective Equipment:LAB COAT, EYE WASH STATION, AND SAFETY
SHOWER.
Work Hygienic Practices:OBSERVE GOOD PERSONAL HYGIENE PRACTICES AND
RECOMMENDED PROCEDURES.
Supplemental Safety and Health

HCC:F4
Spec Gravity:<1
Solubility in Water:COMPLETE
Appearance and Odor:RED LIQUID WITH SLIGHT CHARACTERISTIC ODOR OF
ALCOHOL.
Percent Volatiles by Volume:100

Stability Indicator/Materials to Avoid:YES
STRONG OXIDIZING AGENTS, WATER REACTIVE MATERIALS.
Stability Condition to Avoid:HEAT, SPARKS, FLAME AND OTHER SOURCES OF
IGNITION.
Hazardous Decomposition Products:CARBON MONOXIDE, CARBON DIOXIDE.
Conditions to Avoid Polymerization:WILL NOT OCCUR.

Waste Disposal Methods:DISPOSE OF IN ACCORDANCE WITH LOCAL, STATE, AND
FEDERAL REGULATIONS. ALWAYS CONTACT A PERMITTED WASTE DISPOSER
(TSD) TO ASSURE COMPLIANCE.

Toxicological Information:ETHYL ALCOHOL: LD50(ORAL RAT)= 7,060 MG/KG;
LC50(IHL RAT)= 20,000 PPM/10H. METHYL ISOBUTYL KETONE: LD50 (ORAL
RAT)=5,080 MG/KG; LC50 (IHL-RAT)=8,000 PPM/4H. HEXANE: LD50 (ORAL
RAT)=28,710 MG/KG; TCLO (IHL HUMAN)=5,000 PPM/10M. SAFRANNIN:
NONE. EFFECTS OF OVEREXPOSURE: CHRONIC: SAFRANIN: MUTATION DATA
CITED. ETHYL ALCOHOL: MUTATION DATA CITED. REPRODUCTION EFFECTS
DTA CITED. TUMORIGENIC DATA CITED . METHYL ISOBUTYL KEYTONE: NO
CHRONIC EFFECTS DATA FOUND. HEXANE: MUTATION DATA CITED.
REPRODUCTIVE EFFECTS DATA CITED. EACOF THE 4 INGREDIENTS IS NOT
LISTED AS CAUSING CANCER BY IARC, NTP, OR OSHA.

Ecological:EPA TSCA WASTE NUMBERS: NONE.

Transport Information:NON-REGULATED.

SARA Title III Information:HAZARD CATEGORY FOR SARA SECTION 311/312
REPORTING: ACUTE. SAFRANIN, ETHYL ALCOHOL, HEXANE: SARA EHS SEC.
302 TPQ-NO;SARASEC. 313 CHEMICALS, NAME LIST- NO, CHEMICAL
CATEGORY-NO. CERCLA SEC/ 103 RQ, LB S-NO. RCRA SEC. 261.33-NO.
METHYL ISOBUTYL: ALL ABOVE CTEGORIES -NO EXCEPT CERCLA SEC.103
-RQ=5000 LBS AND RCA SEC. 261.33 -YES.
Federal Regulatory Information:EPA TSCA STATUS: ON TSCA INVENTORY.

Disclaimer (provided with this information by the compiling agencies):
This information is formulated for use by elements of the Department
of Defense. The United States of America in no manner whatsoever,
expressly or implied, warrants this information to be accurate and
disclaims all liability for its use. Any person utilizing this
document should seek competent professional advice to verify and
assume responsibility for the suitability of this information to their
particular situation.